The washing liquid pat dry on the absorbent paper
Pre-detection preparations Syringe Installed Machine repeated freezing and thawing, the cells are destroyed and the intracellular components are released.4), and the cell concentration will reach about 1 million/ml. Collect the supernatant carefully. Urine from a new type of African swine fever tester: collect it in a sterile tube and centrifuge for about 20 minutes (2000-3000 rpm). Quickly freeze and store with liquid nitrogen for later use. Centrifuge for about 20 minutes (2000-3000 rpm).5-10uL, 2-20uL, 20-200uL, 200-1000uL 3. Test equipment required for the experiment 1.
High-precision sampler and pipette tip: 0. 2. Collect the supernatant carefully. 2. 4. Tissue specimen of the new type of African swine fever tester: After cutting the specimen, weigh it. answer. Add 50;L of stop solution to each well, and measure the OD value of each well at 450nm wavelength with the new type African swine fever tester within 15min. If the test cannot be performed immediately, the specimen can be stored at -20°C, but repeated freezing and thawing should be avoided. Discard the liquid, pat dry on absorbent paper, fill each hole with the washing liquid of the new African swine fever tester, let stand for 1 min
, shake off the washing liquid, pat dry on the absorbent paper, and repeat washing the plate 5 times (can also be washed Plate machine washing plate). If precipitation forms during storage, centrifuge again. 5. Extract as soon as possible after the specimen is collected. Collect the supernatant carefully. Add a certain amount of PBS, pH 7. 5. 6. Prepare 37℃ constant temperature Box or water bath 4. 3, need to do 20; washing buffer dilution: distilled water is diluted 1:20, that is, 1 part 20; washing buffer plus 19 parts distilled water. 3.
2. Add 50μL of substrate A and B each to each well, and incubate in a water bath at 37°C, protected from light, for 15 minutes. Operation steps of the new type of African swine fever tester 1. 7. In the use of low-speed centrifuges, how to operate correctly is the key. Next, the prevention and control of the African swine fever epidemic and the restoration of pig production will be the key tasks. Can not detect samples containing NaN3, because NaN3 inhibits horseradish peroxidase (HRP) activity. Centrifuge for about 20 minutes (2000-3000 rpm)
